a Record control number WOS:000283939000015

a ISSN 0196-9781

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QV 4

b Item number .E53 2010

a Personal name Bulbul, Mehmet.

h Medium Electronic Document

a Title Endogenous orexin-A modulates gastric motility by peripheral mechanisms in rats

c Statement of responsibility, etc Mehmet Bulbul, Ruken Tan, Burcu Gemici, Sebahat Ozdem, Ismail Ustunel, Nuray Acar, V. Nimet Izgut-Uysal.

a Place of publication, distribution, etc Elsevier Science Inc.,

c Date of publication, distribution, etc 2010.

b Name of publisher, distributor, etc New York

a Summary, etc Orexin-A (OXA) and orexin receptor type 1 (OX1R) are found in enteric nervous system and smooth muscle cells in the digestive tract. Fasting is a stimulant for OXA synthesis. The aim of the present study was to investigate central and peripheral effects of endogenous OXA on gastric motility. Endogenous OXA synthesis was induced by 36 h fasting. Vagotomy was used to evaluate N.vagus-mediated effects of OXA. Gastric emptying and interdigestive gastric motility were measured by spectrophotometric and manometric methods, respectively. Rats were pretreated with OX1R antagonist SB-334867 prior to measurements. Plasma OXA concentration was assayed with radioimmunoassay while preproorexin (PPO) expression was determined with Western blotting in gastric and hypothalamic tissues. OXA immunoreactivity in antrum was determined with immunohistochemistry. Plasma OXA level, PPO protein expression and OXA immunoreactivity were significantly increased in response to 36 h fasting. Endogenous OXA facilitated gastric emptying and inhibited gastric interdigestive motility. As these effects were abolished with SB-334867, it is likely that gastrokinetic effects of OXA are mediated via OX1R. Vagotomy did not alter OXA-mediated effects. According to current data, OXA is up-regulated both centrally and peripherally upon fasting. Endogenous OXA accelerates gastric emptying while it inhibits interdigestive motility. (C) 2010 Elsevier Inc. All rights reserved.

a Topical term or geographic name as entry element Pharmacology

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Fasting

a Topical term or geographic name as entry element Gastric motility

a Topical term or geographic name as entry element Orexin-A

a Topical term or geographic name as entry element Migrating Myoelectric Complex

a Topical term or geographic name as entry element Vasoactive-Intestinal-Peptide

g Relationship information JUN 2010, Vol. 31 Issue 6, p1099-1108

x International Standard Serial Number 10991108

t Title Reptides

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.peptides.2010.03.007